Mountain bike enthusiasts will find Mugello the perfect spot. The natural environment, characterized by mountains and hills, is well suited to this sport and provides the excursionist the opportunity to reach the most unexpected and fascinating corners of the SO.F.T. (Florence Springs Trekking) and CAI (Italian Alpine Club) routes. In fact, the main SO.F.T. trail, with the alternative paths set out for horseback riding, was created especially for excursions by mountain bike.

The National Railway System offers a special Florence-Faenza line called TRENO+BICI in which certain railway cars allow bikes on board.